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Title 49

Displaying title 49, up to date as of 8/06/2026. Title 49 was last amended 8/04/2026.
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Title 49TransportationPart / Section
Subtitle AOffice of the Secretary of Transportation1 – 99
Part 40Procedures for Transportation Workplace Drug and Alcohol Testing Programs40.1 – 40.413
Subpart AAdministrative Provisions40.1 – 40.7
Subpart BEmployer Responsibilities40.11 – 40.27
Subpart CSpecimen Collection Personnel40.31 – 40.36
Subpart DCollection Sites, Forms, Equipment and Supplies Used in DOT Urine and Oral Fluid Collections40.40 – 40.51
Subpart ESpecimen Collections40.61 – 40.79
Subpart FDrug Testing Laboratories40.81 – 40.111
Subpart GMedical Review Officers and the Verification Process40.121 – 40.167
Subpart HSplit Specimen Tests40.171 – 40.187
Subpart IProblems in Drug Tests40.191 – 40.210
Subpart JAlcohol Testing Personnel40.211 – 40.215
Subpart KTesting Sites, Forms, Equipment and Supplies Used in Alcohol Testing40.221 – 40.235
Subpart LAlcohol Screening Tests40.241 – 40.247
Subpart MAlcohol Confirmation Tests40.251 – 40.255
Subpart NProblems in Alcohol Testing40.261 – 40.277
Subpart OSubstance Abuse Professionals and the Return-to-Duty Process40.281 – 40.311
Subpart PConfidentiality and Release of Information40.321 – 40.333
Subpart QRoles and Responsibilities of Service Agents40.341 – 40.355
Subpart RPublic Interest Exclusions40.361 – 40.413
Appendix A to Part 40
DOT Standards for Urine Collection Kits
Appendix B to Part 40
Oral Fluid Collection Kit Contents
Appendix C to Part 40 [Reserved]
Appendix D to Part 40
DOT Drug Testing Semi-Annual Laboratory Report to Employers
Appendix E to Part 40
Drug Testing Semi-Annual Laboratory Report to DOT
Appendix F to Part 40
Report Format: Split Specimen Failure To Reconfirm
Appendix G to Part 40
SAP Equivalency Requirements for Certification Organizations
Appendix H to Part 40
Drug and Alcohol Testing Information that C/TPAs May Transmit to Employers
Appendix I to Part 40
Alcohol Testing Form
Appendix J to Part 40
DOT Drug and Alcohol Testing Management Information System (MIS) Data Collection Form