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Title 40Chapter ISubchapter DPart 141Subpart C → Appendix


Title 40: Protection of Environment
PART 141—NATIONAL PRIMARY DRINKING WATER REGULATIONS
Subpart C—Monitoring and Analytical Requirements


Appendix A to Subpart C of Part 141—Alternative Testing Methods Approved for Analyses Under the Safe Drinking Water Act

Only the editions stated in the following table are approved.

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.21(f)(3)

OrganismMethodologySM 21st Edition1SM 22nd Edition28SM Online3Other
Total ColiformsTotal Coliform Fermentation Technique9221 A, B9221 A, B9221 A,B-06
   Total Coliform Membrane Filter Technique9222 A, B, C
   Presence-Absence (P-A) Coliform Test9221 D
   ONPG-MUG Test92239223 B9223 B-04
   ColitagTMModified ColitagTM13
   Tecta EC/TC33

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.21(f)(5)

OrganismMethodologySM 22nd Edition28SM Online3
Fecal ColiformsFecal Coliform Procedure9221 E9221 E-06

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.21(f)(6)

OrganismMethodologySM 20th
Edition6
SM 21st
Edition1
SM 22nd
Edition28
SM Online3Other
E.coliONPG-MUG Test9223 B9223 B9223 B9223 B-97, B-04
   ColitagTMModified ColitagTM13
   Tecta EC/TC33

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.23(k)(1)

ContaminantMethodologyEPA methodSM 21st
edition1
SM 22nd
edition28
SM 23rd
edition49
SM Online3ASTM4Other
AlkalinityTitrimetric2320 B2320 B2320 BD1067-06 B, 11 B, 16 B
AntimonyHydride—Atomic AbsorptionD 3697-07, -12
   Atomic Absorption; Furnace3113 B3113 B3113 B3113 B-04, B-10
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
ArsenicAtomic Absorption; Furnace3113 B3113 B3113 B3113 B-04, B-10D 2972-08 C, -15 C
   Hydride Atomic Absorption3114 B3114 B3114 B3114 B-09D 2972-08 B, -15 B
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
BariumInductively Coupled Plasma3120 B3120 B3120 B
   Atomic Absorption; Direct3111 D3111 D3111 D
   Atomic Absorption; Furnace3113 B3113 B3113 B3113 B-04, B-10
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
BerylliumInductively Coupled Plasma3120 B3120 B3120 B
   Atomic Absorption; Furnace3113 B3113 B3113 B3113 B-04, B-10D 3645-08 B, -15 B
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
CadmiumAtomic Absorption; Furnace3113 B3113 B3113 B3113 B-04, B-10
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
CalciumEDTA titrimetric3500-Ca B3500-Ca B3500-Ca BD 511-09, -14 A
   Atomic Absorption; Direct Aspiration3111 B3111 B3111 BD 511-09, -14 B
   Inductively Coupled Plasma3120 B3120 B3120 B
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
   Ion ChromatographyD 6919-09
ChromiumInductively Coupled Plasma3120 B3120 B3120 B
   Atomic Absorption; Furnace3113 B3113 B3113 B3113 B-04, B-10
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
CopperAtomic Absorption; Furnace3113 B3113 B3113 B3113 B-04, B-10D 1688-07, -12 C
   Atomic Absorption; Direct Aspiration3111 B3111 B3111 BD 1688-07, -12 A
   Inductively Coupled Plasma3120 B3120 B3120 B
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
   ColorimetryHach Method 8026;35 Hach Method 10272.36
ConductivityConductance2510 B2510 B2510 BD 1125-14 A
CyanideManual Distillation with MgCl2 followed by:4500-CN C4500-CN C4500-CN C4500-CN C-99D 2036-06 A
   Spectrophotometric, Amenable4500-CN G4500-CN G4500-CN GD 2036-06 B
   Spectrophotometric Manual4500-CN E4500-CN E4500-CN ED2036-06 A
   Selective Electrode4500-CN F4500-CN F4500-CN F
   Gas Chromatography/Mass Spectrometry HeadspaceME355.01.7
FluorideIon Chromatography4110 B4110 B4110 BD 4327-11
   Manual Distillation; Colorimetric SPADNS4500-F B, D4500-F B, D4500-F B, D
   Manual Electrode4500-F C4500-F C4500-F CD 1179-04, 10 B, 16 B
   Automated Alizarin4500-F E4500-F E4500-F E
   Arsenite-Free Colorimetric SPADNSHach SPADNS 2 Method 10225.22
LeadAtomic Absorption; Furnace3113 B3113 B3113 B3113 B-04, B-10D 3559-08 D, 15 D
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
MagnesiumAtomic Absorption3111 B3111 B3111 BD 511-09, -14 B
   Inductively Coupled Plasma3120 B3120 B3120 B
   Complexation Titrimetric Methods3500-Mg B3500-Mg B3500-Mg BD 511-09, -14 A
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
   Ion ChromatographyD 6919-09
MercuryManual, Cold Vapor3112 B3112 B3112 B3112 B-09D 3223-12
NickelInductively Coupled Plasma3120 B3120 B3120 B
   Atomic Absorption; Direct3111 B3111 B3111 B
   Atomic Absorption; Furnace3113 B3113 B3113 B3113 B-04, B-10
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
NitrateIon Chromatography4110 B4110 B4110 BD 4327-11
   Automated Cadmium Reduction4500-NO3 F4500-NO3 F4500-NO3 F
   Manual Cadmium Reduction4500-NO3 E4500-NO3 E4500-NO3 E
   Ion Selective Electrode4500-NO3 D4500-NO3 D4500-NO3 D
   Reduction/ColorimetricSystea Easy
(1-Reagent);8 NECi Nitrate-Reductase.40
   Colorimetric; DirectHach TNTplusTM 835/836 Method 10206.23
   Capillary Ion ElectrophoresisD 6508-15
NitriteIon Chromatography4110 B4110 B4110 BD 4327-11
   Automated Cadmium Reduction4500-NO3 F4500-NO3 F4500-NO3 F
   Manual Cadmium Reduction4500-NO3 E4500-NO3 E4500-NO3 E
   Spectrophotometric4500-NO2 B4500-NO2 B4500-NO2 B
   Reduction/ColorimetricSystea Easy (1-Reagent);8 NECi Nitrate-Reductase.40
   Capillary Ion ElectrophoresisD 6508-15
Ortho-phosphateIon Chromatography4110 B4110 B4110 BD 4327-11
   Colorimetric, ascorbic acid, single reagent4500-P E4500-P E4500-P E4500-P E-99
   Colorimetric, Automated, Ascorbic Acid4500-P F4500-P F4500-P F4500-P F-99Thermo Fisher Discrete Analyzer.41
   Capillary Ion ElectrophoresisD 6508-15
pHElectrometric150.3484500-H+ B4500-H+ B4500-H+ BD 1293-12
SeleniumHydride-Atomic Absorption3114 B3114 B3114 B3114 B-09D 3859-08 A, -15 A
   Atomic Absorption; Furnace3113 B3113 B3113 B3113 B-04, B-10D 3859-08 B, -15 B
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
SilicaColorimetricD859-05, 10, 16
   Molybdosilicate4500-SiO2 C4500-SiO2 C4500-SiO2 C
   Heteropoly blue4500-SiO2 D4500-SiO2 D4500-SiO2 D
   Automated for Molybdate-reactive Silica4500-SiO2 E4500-SiO2 E4500-SiO2 E
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
   Inductively Coupled Plasma3120 B3120 B3120 B
SodiumAtomic Absorption; Direct Aspiration3111 B3111 B3111 B
   Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.22
   Ion ChromatographyD 6919-09
TemperatureThermometric2550255025502550-10

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.24(e)(1)

ContaminantMethodologyEPA methodSM 21st
edition1
SM 22nd
edition,28 SM 23rd
edition49
SM online3
BenzenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
Carbon tetrachloridePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
ChlorobenzenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
1,2-DichlorobenzenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
1,4-DichlorobenzenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
1,2-DichloroethanePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
cis-DichloroethylenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
trans-DichloroethylenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
DichloromethanePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
1,2-DichloropropanePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
EthylbenzenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
StyrenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,;9 524.4.29
TetrachloroethylenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
1,1,1-TrichloroethanePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
TrichloroethylenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
ToluenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
1,2,4-TrichlorobenzenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
1,1-DichloroethylenePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
1,1,2-TrichlorethanePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
Vinyl chloridePurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
Xylenes (total)Purge &Trap/Gas Chromatography/Mass Spectrometry524.3,9 524.4.29
2,4-DGas Chromatography/Electron Capture Detection (GC/ECD)6640 B6640 B6640 B-01, B-06.
2,4,5-TP (Silvex)Gas Chromatography/Electron Capture Detection (GC/ECD)6640 B6640 B6640 B-01, B-06.
AlachlorSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
AtrazineLiquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC/ESI-MS/MS)536.25
   Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3,24523.26
Benzo(a)pyreneSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
CarbofuranHigh-performance liquid chromatography (HPLC) with post-column derivatization and fluorescence detection6610 B6610 B6610 B-04.
ChlordaneSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
DalaponIon Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS)557.14
   Gas Chromatography/Electron Capture Detection (GC/ECD)6640 B6640 B6640 B-01, B-06.
Di(2-ethylhexyl)adipateSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
Di(2-ethylhexyl)phthalateSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
Dibromochloropropane (DBCP)Purge &Trap/Gas Chromatography/Mass Spectrometry524.3.9
DinosebGas Chromatography/Electron Capture Detection (GC/ECD)6640 B6640 B6640 B-01, B-06.
EndrinSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
Ethyl dibromide (EDB)Purge &Trap/Gas Chromatography/Mass Spectrometry524.3.9
GlyphosateHigh-Performance Liquid Chromatography (HPLC) with Post-Column Derivatization and Fluorescence Detection6651 B6651 B6651 B-00, B-05.
HeptachlorSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
Heptachlor EpoxideSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
HexachlorobenzeneSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
Hexachlorocyclo-pentadieneSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
LindaneSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
MethoxychlorSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
OxamylHigh-performance liquid chromatography (HPLC) with post-column derivatization and fluorescence detection6610 B6610 B6610 B-04.
PCBs (as Aroclors)Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
PentachlorophenolGas Chromatography/Electron Capture Detection (GC/ECD)6640 B6640 B6640 B-01, B-06.
Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
PicloramGas Chromatography/Electron Capture Detection (GC/ECD)6640 B6640 B6640 B-01, B-06.
SimazineLiquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC/ESI-MS/MS)536.25
   Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3,24523.26
ToxapheneSolid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)525.3.24
Total TrihalomethanesPurge &Trap/Gas Chromatography/Mass Spectrometry524.3,9524.429

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.25(a)

ContaminantMethodologyEPA
method
SM 21st
edition1
SM 22nd
edition,28
SM 23rd
edition49
ASTM4SM Online3
Naturally Occurring:
Gross alpha and betaEvaporation900.0, Rev. 1.0507110 B7110 B
  Liquid ScintillationD 7283-177110 D-17.
Gross alphaCoprecipitation7110 C7110 C
Radium 226Radon emanation7500-Ra C7500-Ra CD 3454-05
   Radiochemical7500-Ra B7500-Ra BD 2460-07
   Gamma Spectrometry7500-Ra E7500-Ra E-07.
Radium 228Radiochemical7500-Ra D7500-Ra D
   Gamma Spectrometry7500Ra-E7500-Ra E-07.
UraniumRadiochemical7500-U B7500-U B
   ICP-MS3125D 5673-05, 10, 16
   Alpha spectrometry7500-U C7500-U CD 3972-09
   Laser PhosphorimetryD 5174-07
   Alpha Liquid Scintillation SpectrometryD 6239-09
Man-Made:
Radioactive CesiumRadiochemical7500-Cs B7500-Cs B
   Gamma Ray Spectrometry71207120D 3649-06
Radioactive IodineRadiochemical7500-I B7500-I BD 3649-06
         7500-I C7500-I C
         7500-I D7500-I D
   Gamma Ray Spectrometry71207120D 4785-08
Radioactive Strontium 89, 90Radiochemical7500-Sr B7500-Sr B
TritiumLiquid Scintillation7500-3H B7500-3H BD 4107-08
Gamma EmittersGamma Ray71207120D 3649-06
   Spectrometry7500-Cs B7500-Cs BD 4785-08
   7500-I B7500-I B

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.74(a)(1)

OrganismMethodologySM 21st
edition1
SM 22nd
edition28
SM 23rd
edition49
SM Online3Other
Total ColiformTotal Coliform Fermentation Technique9221 A, B, C9221 A, B, C9221 A, B, C9221 A,B,C-06
   Total Coliform Membrane Filter Technique9222 A, B, C9222 A, B, C.
   ONPG-MUG Test92239223 B9223 B9223 B-04.
Fecal ColiformsFecal Coliform Procedure9221 E9221 E9221 E9221 E-06.
   Fecal Coliform Filter Procedure9222 D9222 D9222 D9222 D-06.
Heterotrophic bacteriaPour Plate Method9215 B9215 B9215 B9215 B-04.
TurbidityNephelometric Method2130 B2130 B2130 BHach Method 8195, Rev. 3.0.52
   Laser Nephelometry (on-line)Mitchell M5271,10 Mitchell M5331, Rev. 1.2,42 Lovibond PTV 6000.46
   LED Nephelometry (on-line)Mitchell M5331,11
Mitchell M5331, Rev. 1.2,42 Lovibond PTV 2000.45
   LED Nephelometry (on-line)AMI Turbiwell,15 Lovibond PTV 1000.44
   LED Nephelometry (portable)Orion AQ4500.12
   360° NephelometryHach Method 10258 Rev. 1.0,39 Hach Method 10258, Rev. 2.0.51

Alternative Testing Methods for Disinfectant Residuals Listed at 40 CFR 141.74(a)(2)

ResidualMethodologySM 21st
edition1
SM 22nd edition,28 SM 23rd
edition49
ASTM4Other
Free ChlorineAmperometric Titration4500-Cl D4500-Cl DD 1253-08, -14
   DPD Ferrous Titrimetric4500-Cl F4500-Cl F
   DPD Colorimetric4500-Cl G4500-Cl GHach Method 10260.31
   Indophenol ColorimetricHach Method 10241.34
   Syringaldazine (FACTS)4500-Cl H4500-Cl H
   On-line Chlorine AnalyzerEPA 334.0.16
   Amperometric SensorChloroSense.17
Total ChlorineAmperometric Titration4500-Cl D4500-Cl DD 1253-08, -14
   Amperometric Titration (Low level measurement)4500-Cl E4500-Cl E.
   DPD Ferrous Titrimetric4500-Cl F4500-Cl F.
   DPD Colorimetric4500-Cl G4500-Cl GHach Method 10260.31
   Iodometric Electrode4500-Cl I4500-Cl I.
   On-line Chlorine AnalyzerEPA 334.0.16
   Amperometric SensorChloroSense.17
Chlorine DioxideAmperometric Titration4500-ClO2 C4500-ClO2 C.
   Amperometric Titration4500-ClO2 E.4500-ClO2 E.
   Amperometric SensorChlordioX Plus.32
OzoneIndigo Method4500-O3 B4500-O3 B.

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.131(b)(1)

ContaminantMethodologyEPA methodASTM4SM online3SM 21st edition1SM 22nd edition,28
SM 23rd edition49
Other
TTHMP&T/GC/MS524.3,9 524.4.29
HAA5LLE (diazomethane)/GC/ECD6251 B-076251 B6251 B.
   Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS)557.14
   Two-Dimensional Ion Chromatography (IC) with Suppressed Conductivity DetectionThermo Fisher 557.1.47
BromateTwo-Dimensional Ion Chromatography (IC)302.0.18
   Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS)557.14
   Chemically Suppressed Ion ChromatographyD 6581-08 A.
   Electrolytically Suppressed Ion ChromatographyD 6581-08 B.
ChloriteChemically Suppressed Ion ChromatographyD 6581-08 A.
   Electrolytically Suppressed Ion ChromatographyD 6581-08 B.
Chlorite—daily monitoring as prescribed in 40 CFR 141.132(b)(2)(i)(A)Amperometric Titration4500-ClO2 E4500-ClO2 E.
   Amperometric SensorChlordioX Plus.32

Alternative Testing Methods for Disinfectant Residuals Listed at 40 CFR 141.131(c)(1)

ResidualMethodologySM 21st
edition1
SM 22nd
edition,28
SM 23rd
edition49
ASTM4Other
Free ChlorineAmperometric Titration4500-Cl D4500-Cl DD 1253-08, -14.
   DPD Ferrous Titrimetric4500-Cl F4500-Cl F
   DPD Colorimetric4500-Cl G4500-Cl GHach Method 10260.31
   Indophenol ColorimetricHach Method 10241.34
   Syringaldazine (FACTS)4500-Cl H4500-Cl H.
   Amperometric SensorChloroSense.17
   On-line Chlorine AnalyzerEPA 334.0.16
Combined ChlorineAmperometric Titration4500-Cl D4500-Cl DD 1253-08, -14.
   DPD Ferrous Titrimetric4500-Cl F4500-Cl F
   DPD Colorimetric4500-Cl G4500-Cl GHach Method 10260.31
Total ChlorineAmperometric Titration4500-Cl D4500-Cl DD 1253-08, -14.
   Low level Amperometric Titration4500-Cl E4500-Cl E.
   DPD Ferrous Titrimetric4500-Cl F4500-Cl F.
   DPD Colorimetric4500-Cl G4500-Cl GHach Method 10260.31
   Iodometric Electrode4500-Cl I4500-Cl I.
   Amperometric SensorChloroSense.17
   On-line Chlorine AnalyzerEPA 334.0.16
Chlorine DioxideAmperometric Method II4500-ClO2 E4500-ClO2 E.
   Amperometric SensorChlordioX Plus.32

Alternative Testing Methods for Disinfectant Residuals Listed at 40 CFR 141.131(c)(2), If Approved by the State

ResidualMethodologyMethod
Free ChlorineTest StripsMethod D99-0035

Alternative Testing Methods for Parameters Listed at 40 CFR 141.131(d)

ParameterMethodologySM 21st edition1SM 22nd edition28SM 23rd edition49SM online3EPAOther
Total Organic Carbon (TOC)High Temperature Combustion5310 B5310 B5310 B415.3, Rev 1.2.19
   Persulfate-Ultraviolet or Heated Persulfate Oxidation5310 C5310 C5310 C415.3, Rev 1.2.19Hach Method 10267.38
   Wet Oxidation5310 D5310 D415.3, Rev 1.2.19
   Ozone OxidationHach Method 10261.37
Specific Ultraviolet Absorbance (SUVA)Calculation using DOC and UV254 data415.3, Rev 1.2.19
Dissolved Organic Carbon (DOC)High Temperature Combustion5310 B5310 B5310 B415.3, Rev 1.2.19
   Persulfate-Ultraviolet or Heated Persulfate Oxidation5310 C5310 C5310 C415.3, Rev 1.2.19
   Wet Oxidation5310 D5310 D415.3, Rev 1.2.19
Ultraviolet absorption at 254 nm (UV254)Spectrophotometry5910 B5910 B5910 B5910 B-11415.3, Rev 1.2.19

Alternative Testing Methods With MRL ≤0.0010 mg/L for Monitoring Listed at 40 CFR 141.132(b)(3)(ii)(B)

ContaminantMethodologyEPA method
BromateTwo-Dimensional Ion Chromatography (IC)302.018
   Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS)55714

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.402(c)(2)

OrganismMethodologySM 20th
edition6
SM 21st
edition1
SM 22nd
edition28
SM 23rd
edition49
SM online3Other
E. coliColilert9223 B9223 B9223 B9223 B-97, B-04.
   Colisure9223 B9223 B9223 B9223 B-97, B-04.
   Colilert-189223 B9223 B9223 B9223 B9223 B-97, B-04.
   Readycult®Readycult®.20
   ColitagModified Colitag.TM13
   Chromocult®Chromocult®.21
   EC-MUG9221 F9221 F9221 F-06.
   NA-MUG9222 I.
   m-ColiBlue24 Test9222 J.
   Tecta EC/TC 3343
EnterococciMultiple-Tube Technique9230 B-04.
   Membrane Filter Techniques9230 C.
   Fluorogenic Substrate Enterococcus Test (using Enterolert)9230 D.
ColiphageTwo-Step Enrichment Presence-Absence ProcedureFast Phage.30

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.704(a)

OrganismMethodologyEPA Method
CryptosporidiumFiltration/Immunomagnetic Separation/Immunofluorescence Assay Microscopy1623.127

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.704(b)

OrganismMethodologySM 20th edition6
E. coliMembrane Filtration, Two Step9222 D/9222 G

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.852(a)(5)

OrganismMethodology categoryMethodSM 20th, 21st
editions 1 6
SM 22nd
edition28
SM 23rd
edition49
SM online3
Total ColiformsLactose Fermentation MethodsStandard Total Coliform Fermentation Technique9221 B.1, B.29221 B.1, B.2, B.3, B.49221 B.1, B.2-06.
   Presence-Absence (P-A) Coliform Test9221 D.1, D.2, D.3
   Membrane Filtration MethodsStandard Total Coliform Membrane Filter Procedure using Endo Media9222 B, C.
   Simultaneous Detection of Total Coliforms and E. coli by Dual Chromogen Membrane Filter Procedure (using mColiBlue24 medium)9222 J.
   Enzyme Substrate MethodsColilert®9223 B9223 B9223 B-04.
   Colisure®9223 B9223 B9223 B-04.
   Colilert-189223 B9223 B9223 B9223 B-04.
   Tecta EC/TC 33 43
Escherichia coliEscherichia coli Procedure (following Lactose Fermentation Methods)EC-MUG medium9221 F.19221 F.19221 F.1-06.
   Escherichia coli Partitioning Methods (following Membrane Filtration Methods)EC broth with MUG (EC-MUG)9222 H.
   NA-MUG medium9222 I.
   Simultaneous Detection of Total Coliforms and E. coli by Dual Chromogen Membrane Filter ProceduremColiBlue24 medium9222 J.
   Enzyme Substrate MethodsColilert®9223 B9223 B9223 B-04.
   Colisure®9223 B9223 B9223 B-04.
   Colilert-189223 B9223 B9223 B9223 B-04.
   Tecta EC/TC 33 43

Alternative Testing Methods for Contaminants Listed at 40 CFR 143.4(b)

ContaminantMethodologyEPA methodASTM4SM 21st
edition1
SM 22nd
edition,28
SM 23rd
edition49
SM online3
AluminumAxially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.2.2
   Atomic Absorption; Direct3111 D3111 D.
   Atomic Absorption; Furnace3113 B3113 B3113 B-04, B-10.
   Inductively Coupled Plasma3120 B3120 B.
ChlorideSilver Nitrate TitrationD 512-04 B, 12 B4500-Cl B4500-Cl B
   Ion ChromatographyD 4327-114110 B4110 B.
   Potentiometric Titration4500-Cl D4500-Cl D.
ColorVisual Comparison2120 B2120 B.
Foaming AgentsMethylene Blue Active Substances (MBAS)5540 C5540 C.
IronAxially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.2.2
   Atomic Absorption; Direct3111 B3111 B.
   Atomic Absorption; Furnace3113 B3113 B3113 B-04, B-10.
   Inductively Coupled Plasma3120 B3120 B.
ManganeseAxially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.2.2
   Atomic Absorption; Direct3111 B3111 B.
   Atomic Absorption; Furnace3113 B3113 B3113 B-04, B-10.
   Inductively Coupled Plasma3120 B3120 B.
OdorThreshold Odor Test2150 B2150 B.
SilverAxially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.2.2
   Atomic Absorption; Direct3111 B3111 B.
   Atomic Absorption; Furnace3113 B3113 B3113 B-04, B-10.
   Inductively Coupled Plasma3120 B3120 B.
SulfateIon ChromatographyD 4327-114110 B4110 B.
   Gravimetric with ignition of residue4500-SO4 2 C4500-SO4 2 C4500-SO4 2 C-97.
   Gravimetric with drying of residue4500-SO4 2 D4500-SO4 2 D4500-SO4 2 D-97.
   Turbidimetric methodD 516-07, 11, 164500-SO4 2 E4500-SO4 2 E4500-SO4 2 E-97.
   Automated methylthymol blue method4500-SO4 2 F4500-SO4 2 F4500-SO4 2 F-97.
Total Dissolved SolidsTotal Dissolved Solids Dried at 180 deg C2540 C2540 C.
ZincAxially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)200.5, Revision 4.2.2
   Atomic Absorption; Direct Aspiration3111 B3111 B.
   Inductively Coupled Plasma3120 B3120 B.

1Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street, NW., Washington, DC 20001-3710.

2EPA Method 200.5, Revision 4.2. “Determination of Trace Elements in Drinking Water by Axially Viewed Inductively Coupled Plasma-Atomic Emission Spectrometry.” 2003. EPA/600/R-06/115. (Available at http://www.epa.gov/water-research/epa-drinking-water-research-methods.)

3Standard Methods Online are available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.

4Available from ASTM International, 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959 or http://astm.org. The methods listed are the only alternative versions that may be used.

5Method D99-003, Revision 3.0. “Free Chlorine Species (HOCl and OCl) by Test Strip,” November 21, 2003. Available from Industrial Test Systems, Inc., 1875 Langston St., Rock Hill, SC 29730.

6Standard Methods for the Examination of Water and Wastewater, 20th edition (1998). Available from American Public Health Association, 800 I Street, NW., Washington, DC 20001-3710.

7Method ME355.01, Revision 1.0. “Determination of Cyanide in Drinking Water by GC/MS Headspace,” May 26, 2009. Available at http://www.nemi.gov or from James Eaton, H & E Testing Laboratory, 221 State Street, Augusta, ME 04333. (207) 287-2727.

8Systea Easy (1-Reagent). “Systea Easy (1-Reagent) Nitrate Method,” February 4, 2009. Available at http://www.nemi.gov or from Systea Scientific, LLC., 900 Jorie Blvd., Suite 35, Oak Brook, IL 60523.

9EPA Method 524.3, Version 1.0. “Measurement of Purgeable Organic Compounds in Water by Capillary Column Gas Chromatography/Mass Spectrometry,” June 2009. EPA 815-B-09-009. Available at https://www.nemi.gov.

10Mitchell Method M5271, Revision 1.1. “Determination of Turbidity by Laser Nephelometry,” March 5, 2009. Available at http://www.nemi.gov or from Leck Mitchell, PhD, PE, 656 Independence Valley Dr., Grand Junction, CO 81507.

11Mitchell Method M5331, Revision 1.1. “Determination of Turbidity by LED Nephelometry,” March 5, 2009. Available at http://www.nemi.gov or from Leck Mitchell, PhD, PE, 656 Independence Valley Dr., Grand Junction, CO 81507.

12Orion Method AQ4500, Revision 1.0. “Determination of Turbidity by LED Nephelometry,” May 8, 2009. Available at http://www.nemi.gov or from Thermo Scientific, 166 Cummings Center, Beverly, MA 01915, http://www.thermo.com.

13Modified ColitagTM Method, “Modified ColitagTM Test Method for the Simultaneous Detection of E. coli and other Total Coliforms in Water (ATP D05-0035),” August 28, 2009. Available at http://www.nemi.gov or from CPI, International, 580 Skylane Boulevard, Santa Rosa, CA 95403.

14EPA Method 557. “Determination of Haloacetic Acids, Bromate, and Dalapon in Drinking Water by Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS),” September 2009. EPA 815-B-09-012. Available at https://www.nemi.gov.

15AMI Turbiwell, “Continuous Measurement of Turbidity Using a SWAN AMI Turbiwell Turbidimeter,” August 2009. Available at http://www.nemi.gov or from Markus Bernasconi, SWAN Analytische Instrumente AG, Studbachstrasse 13, CH-8340 Hinwil, Switzerland.

16EPA Method 334.0. “Determination of Residual Chlorine in Drinking Water Using an On-line Chlorine Analyzer,” September 2009. EPA 815-B-09-013. Available at https://www.nemi.gov.

17ChloroSense. “Measurement of Free and Total Chlorine in Drinking Water by Palintest ChloroSense,” August 2009. Available at https://www.nemi.gov or from Palintest Ltd, 1455 Jamike Avenue (Suite 100), Erlanger, KY 41018.

18EPA Method 302.0. “Determination of Bromate in Drinking Water using Two-Dimensional Ion Chromatography with Suppressed Conductivity Detection,” September 2009. EPA 815-B-09-014. Available at https://www.nemi.gov.

19EPA 415.3, Revision 1.2. “Determination of Total Organic Carbon and Specific UV Absorbance at 254 nm in Source Water and Drinking Water,” September 2009. EPA/600/R-09/122. Available at http://www.epa.gov/water-research/epa-drinking-water-research-methods.

20Readycult® Method, “Readycult® Coliforms 100 Presence/Absence Test for Detection and Identification of Coliform Bacteria and Escherichia coli in Finished Waters,” January, 2007. Version 1.1. Available from EMD Millipore (division of Merck KGaA, Darmstadt, Germany), 290 Concord Road, Billerica, MA 01821.

21Chromocult® Method, “Chromocult® Coliform Agar Presence/Absence Membrane Filter Test Method for Detection and Identification of Coliform Bacteria and Escherichia coli in Finished Waters,” November, 2000. Version 1.0. EMD Millipore (division of Merck KGaA, Darmstadt, Germany), 290 Concord Road, Billerica, MA 01821.

22Hach Company. “Hach Company SPADNS 2 (Arsenite-Free) Fluoride Method 10225—Spectrophotometric Measurement of Fluoride in Water and Wastewater,” January 2011. 5600 Lindbergh Drive, P.O. Box 389, Loveland, Colorado 80539.

23Hach Company. “Hach Company TNTplusTM 835/836 Nitrate Method 10206—Spectrophotometric Measurement of Nitrate in Water and Wastewater,” January 2011. 5600 Lindbergh Drive, P.O. Box 389, Loveland, Colorado 80539.

24EPA Method 525.3. “Determination of Semivolatile Organic Chemicals in Drinking Water by Solid Phase Extraction and Capillary Column Gas Chromatography/Mass Spectrometry (GC/MS),” February 2012. EPA/600/R-12/010. Available at http://www.epa.gov/water-research/epa-drinking-water-research-methods.

25EPA Method 536. “Determination of Triazine Pesticides and their Degradates in Drinking Water by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC/ESI-MS/MS),” October 2007. EPA 815-B-07-002. Available at the National Service Center for Environmental Publications (EPA Method 536).

26EPA Method 523. “Determination of Triazine Pesticides and their Degradates in Drinking Water by Gas Chromatography/Mass Spectrometry (GC/MS),” February 2011. EPA 815-R-11-002. Available at the National Service Center for Environmental Publications (EPA Method 523).

27EPA Method 1623.1. “Cryptosporidium and Giardia in Water by Filtration/IMS/FA,” 2012. EPA-816-R-12-001. Available at the National Service Center for Environmental Publications (www.epa.gov/nscep). Search “816R12001”.

28Standard Methods for the Examination of Water and Wastewater, 22nd edition (2012). Available from American Public Health Association, 800 I Street NW., Washington, DC 20001-3710.

29EPA Method 524.4, Version 1.0. “Measurement of Purgeable Organic Compounds in Water by Gas Chromatography/Mass Spectrometry using Nitrogen Purge Gas,” May 2013. EPA 815-R-13-002. Available at the National Service Center for Environmental Publications (EPA Method 524.4).

30Charm Sciences Inc. “Fast Phage Test Procedure. Presence/Absence for Coliphage in Ground Water with Same Day Positive Prediction”. Version 009. November 2012. 659 Andover Street, Lawrence, MA 01843. Available at www.charmsciences.com.

31Hach Company. “Hach Method 10260—Determination of Chlorinated Oxidants (Free and Total) in Water Using Disposable Planar Reagent-filled Cuvettes and Mesofluidic Channel Colorimetry,” April 2013. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

32ChlordioX Plus. “Chlorine Dioxide and Chlorite in Drinking Water by Amperometry using Disposable Sensors,” November 2013. Available from Palintest Ltd, Jamike Avenue (Suite 100), Erlanger, KY 41018.

33Tecta EC/TC. “TechtaTMEC/TC Medium and TechtaTMInstrument: A Presence/Absence Method for the Simultaneous Detection of Total Coliforms and Escherichia coli (E. coli) in Drinking Water,” version 1.0, May 2014. Available from Pathogen Detection Systems, Inc., 382 King Street East, Kingston, Ontario, Canada, K7K 2Y2.

34Hach Company. “Hach Method 10241—Spectrophotometric Measurement of Free Chlorine (Cl2) in Drinking Water,” November 2015. Revision 1.2. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

35Hach Company. “Hach Method 8026—Spectrophotometric Measurement of Copper in Finished Drinking Water,” December 2015. Revision 1.2. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

36Hach Company. “Hach Method 10272—Spectrophotometric Measurement of Copper in Finished Drinking Water,” December 2015. Revision 1.2. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

37Hach Company. “Hach Method 10261—Total Organic Carbon in Finished Drinking Water by Catalyzed Ozone Hydroxyl Radical Oxidation Infrared Analysis,” December 2015. Revision 1.2. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

38Hach Company. “Hach Method 10267—Spectrophotometric Measurement of Total Organic Carbon (TOC) in Finished Drinking Water,” December 2015. Revision 1.2. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

39Hach Company. “Hach Method 10258—Determination of Turbidity by 360° Nephelometry,” January 2016. Revision 1.0. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

40Nitrate Elimination Company, Inc. (NECi). “Method for Nitrate Reductase Nitrate-Nitrogen Analysis of Drinking Water,” February 2016. Superior Enzymes, Inc., 334 Hecla Street, Lake Linden, Michigan 49945.

41Thermo Fisher. “Thermo Fisher Scientific Drinking Water Orthophosphate Method for Thermo Scientific Gallery Discrete Analyzer,” February 2016. Revision 5. Thermo Fisher Scientific, Ratastie 2, 01620 Vantaa, Finland.

42Mitchell Method M5331, Revision 1.2. “Determination of Turbidity by LED or Laser Nephelometry,” February 2016. Available from Leck Mitchell, Ph.D., PE, 656 Independence Valley Dr., Grand Junction, CO 81507.

43Tecta EC/TC. “TectaTM EC/TC Medium and the TectaTM Instrument: A Presence/Absence Method for the Simultaneous Detection of Total Coliforms and Escherichia coli (E. coli) in Drinking Water,” version 2.0, February 2017. Available from Pathogen Detection Systems, Inc., 382 King Street East, Kingston, Ontario, Canada, K7K 2Y2.

44Lovibond PTV 1000. “Continuous Measurement of Drinking Water Turbidity Using a Lovibond PTV 1000 White Light LED Turbidimeter,” December 2016. Revision 1.0. Available from Tintometer, Inc., 6456 Parkland Drive, Sarasota, FL 34243.

45Lovibond PTV 2000. “Continuous Measurement of Drinking Water Turbidity Using a Lovibond PTV 2000 660-nm LED Turbidimeter,” December 2016. Revision 1.0. Available from Tintometer, Inc., 6456 Parkland Drive, Sarasota, FL 34243.

46Lovibond PTV 6000. “Continuous Measurement of Drinking Water Turbidity Using a Lovibond PTV 6000 Laser Turbidimeter,” December 2016. Revision 1.0. Available from Tintometer, Inc., 6456 Parkland Drive, Sarasota, FL 34243.

47Thermo Fisher. “Thermo Fisher Method 557.1: Determination of Haloacetic Acids in Drinking Water using Two-Dimensional Ion Chromatography with Suppressed Conductivity Detection,” January 2017. Version 1.0. Available from Thermo Fisher Scientific, 490 Lakeside Dr., Sunnyvale, CA 94085 (Richard.jack@thermofisher.com).

48EPA Method 150.3. “Determination of pH in Drinking Water,” February 2017. EPA 815-B-17-001. Available at the National Service Center for Environmental Publications (EPA Method 150.3).

49Standard Methods for the Examination of Water and Wastewater, 23rd edition (2017). Available from American Public Health Association, 800 I Street NW, Washington, DC 20001-3710.

50EPA Method 900.0, Rev. 1.0. “Determination of Gross Alpha and Gross Beta in Drinking Water,” February 2018. EPA 815-B-18-002. Available at the National Service Center for Environmental Publications (EPA Method 900.0 Rev 1.0).

51Hach Company. “Hach Method 10258—Determination of Turbidity by 360° Nephelometry,” March 2018. Revision 2.0. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

52Hach Company. “Hach Method 8195—Determination of Turbidity by Nephelometry,” March 2018. Revision 3.0. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

[74 FR 38353, Aug. 3, 2009, as amended at 74 FR 57914, Nov. 10, 2009; 74 FR 63069, Dec. 2, 2009; 75 FR 32299, June 8, 2010; 76 FR 37018, June 24, 2011; 77 FR 38527, June 28, 2012; 78 FR 32565, May 31, 2013; 78 FR 37463, June 21, 2013; 79 FR 35086, June 19, 2014; 79 FR 36428, June 27, 2014; 81 FR 46844, July 19, 2016; 82 FR 34867, July 27, 2017; 83 FR 51644, Oct. 12, 2018; 83 FR 54676, Oct. 31, 2018]

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